Fig. 7.

Effect of cytokines on NK cells activation in responding to dengue infected DCs.

PBMCs and DENV-infected autologous DCs were co-cultured with 10 µg/ml of indicated blocking mAbs or isotype-matched control (n = 4–12) for 6 or 24 h. (A) Representative FACS plots demonstrate frequencies of IFN-γ (top) or CD107a (bottom) positive NK cells (CD56⁺CD3⁻) in a presence of blocking mAbs for 24 h. PBMCs co-cultured with mock-treated DCs or R10 and PMA/Ionomycin were used as negative and positive control, respectively. (B) Box and whisker plots demonstrate the frequencies of IFN-γ⁺ NK at 6 h (left) and 24 h (right) stimulation. (C) Box and whisker plots demonstrate the frequencies of CD107a⁺ NK cells at 24 h stimulation. The differences of NK cells responses comparing to isotype control were analyzed by Wilcoxon matched-pairs signed rank test. Asterisks indicated P-values: *P < 0.05, **P < 0.01, ***P < 0.001 and “ns.” indicates a non-statistically significant.