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. 2024 May 18;15:4228. doi: 10.1038/s41467-024-48393-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative 3D whole-brain imaging of mouse, rat, ferret, and marmoset via PATTERN. b, c Geometric analysis of the representative mouse, rat, ferret, or marmoset brain according to data acquired by PATTERN. HY Hypothalamus, HPF Hippocampus Formation, TH Thalamus. d Validation of PATTERN-acquired fluorescence signal. e Dual-color labeling strategy for visualizing projections from the motor cortex to the spinal cord. Partially created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en. f Bright field images of entire central nervous system. 3D-view images by PATTERN of the entire central nervous system (g), brain only (h), medulla oblongata (i), and spinal cord (j). k Coronal section via PATTERN (left column) and widefield microscopy (right column), medulla oblongata (MO), and spinal cord (SPC). Positions were labeled by roman numerals corresponding to (g).