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PCR conditions
| cDNA | Forward primer | Reverse primer | Annealing temperature (°C) | Product length (bp) |
| icIL-1Ra1 | A | C | 55 | 577 |
| sIL-1Ra | B | C | 55 | 521 |
| Unspliced IL1-Ra | B | D | 55 | 303 |
| β-Actin | E | F | 60 | 579 |
| sIL-1Ra (LC) | B | G | 57 | 268 |
| GAPDH (LC) | H | I | 57 | 191 |
LC, Light Cycler™ (Roche Molecular Biochemicals, Basel, Switzerland). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
