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Methyl incorporation by m5U54 methyltransferase. TSLs Ψ55 (closed square), unmodified (open square) and dU55 (closed circle) were used as substrates for m5U54 methyltransferase as described in the text. Aliquots were taken at 10, 20, 30 and 60 min. Moles of methyl group incorporated into TSLs are normalized to moles of methyl group incorporated into TSL Ψ55. Although not pictured, TSLs m1Ψ55, m1m3Ψ55, dU54 and dU54dU55 produced similar results to dU55.
