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. 2000 Mar 15;28(6):1455–1464. doi: 10.1093/nar/28.6.1455

Figure 1.

Figure 1

Structure of the primary constructs pOct1.9 and pOct8.5 and multi-targeting construct pMR. pOct1.9 and pOct8.5 contain a PGKhprt cassette 5′ to a lacZ reporter gene. lacZ expression is directed by 1.9 (pOct1.9) and 8.5 kb (pOct8.5) of 5′-flanking sequence from the Oct4 gene, respectively. In the targeting vector pMR, part of the HPRT gene has been replaced with IRESneo to allow selection for neo expression and against HPRT expression and a c-mos termination sequence to prevent read-through. A multiple cloning site (MCS) was included to allow the insertion of tissue-specific promoters or transgenes. The 5′ and 3′ homology arms are given by the PGK and part of the HPRT gene and by the lacZ gene, respectively. The position of the probes used for Southern blot analysis and the position of the SacI sites are indicated.