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. 2000 Mar 15;28(6):1455–1464. doi: 10.1093/nar/28.6.1455

Figure 2.

Figure 2

lacZ expression in ES cells in vitro. (A) lacZ expression with the randomly integrated constructs pOct1.9 (a) and pOct8.5 (b) in ES cells and their differentiated derivatives in vitro. The percentage of clones staining in ES cells only, in differentiated cells only, staining in both ES and differentiated cells and with no staining is given in histogram format (a) for clones transfected with pOct1.9 and (b) for clones transfected with pOct8.5. (B) Monolayer cultures of clone 91 (a and b) and clone 710 (c and d) grown as undifferentiated ES cells (a and c) and following differentiation (b and d). Clones 91 and 710 contain the constructs pOct1.9 and pOct8.5, respectively. Down-regulation of lacZ expression following differentiation of ES cells is apparent in clone 710 (c and d), while in clone 91 lacZ is expressed in both undifferentiated and differentiated cells (a and b).