. 2000 Mar 15;28(6):1455–1464. doi: 10.1093/nar/28.6.1455

Table 1. Targeting efficiencies at two random sites.

Targeting	Site 91 (pOct1.9)				Site 710 (Oct8.5)
constructs	G4l8^R colonies^a	G4l8^R/6TG^R colonies	G4l8^R/6TG^R G4l8^R	Correctly targeted^b G4l8^R/6TG^R	G4l8^R colonies^a	G4l8^R/6TG^R colonies	G4l8^R/6TG^R G4l8^R	Correctly targeted^b G4l8^R/6TG^R
TG	nd	208	nd	83%	3312	3	9 × 10^–4	50%
Myf	3664	27	7.4 × 10^–3	73%	3808	13	3.4 × 10^–3	73%
Hox 2.6A	5184	25	4.8 × l0^–3	54%	3392	8	2.4 × 10^–3	86%

Numbers of G418-resistant and doubly (G418 and 6TG) resistant colonies in each of three targeting experiments. Adjusted targeting frequency reflects the proportion of doubly resistant clones which, on average, were correctly targeted and gave the predicted bands upon Southern analysis of both the 5′- and 3′-flanks. nd, not determined.

^aDrug-resistant colonies per 2 × 10⁷ cells.

^bAs determined by Southern blot analysis.