Table 1. A summary of the nuclear and mitochondrial genotypes of the different compatible strains used in the vegetative fusion experiments.
Strain | Nuclear | Mitochondrial |
---|---|---|
L1 | Hygromycin-resistant | cytb-i2(l) |
L2 | Phleomycin-resistant | cytb-i2(–) |
L3 | Phleomycin-resistant | cytb-i2(s) |
L4 | Hygromycin-resistant | cytb-i2(–) |
These strains were constructed by nuclear transformation of the L isolate with plasmid pBC phleo or pBC hygro, which confer resistance to phleomycin and hygromycin, respectively. After transformation, protoplast fusions with incompatible strains of P.curvicolla A and L allowed us to introduce the different forms of the cytb-i2 intron into their mtDNA (see Materials and Methods). Cytb-i2(l) and cytb-i2(s) indicate that the mitochondrial genome contains the long or short form of the intron, respectively, and cytb-i2(–) that it lacks it.