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. 2024 May 10;13(5):bio060338. doi: 10.1242/bio.060338

Fig. 4.

Fig. 4.

The incorporation of CD9 in Rab5 endosomes is inhibited in ADAP1 and ARAP1KD cells. (A) MCF7 cells were transfected with each siRNA as indicated, then transfected with GFP-Rab5Q79L (green), and stained with anti-CD9 antibody (red). The insets were enlarged, and Rab5 endosomes are indicated with arrowheads. CD9 is localized in endosomes in control cells, while in ADAP1 and ARAP1KD cells, CD9 localization in endosomes was decreased. (B) The experiment in A was repeated three times. More than 15 cells were counted per experiment and more than 45 cells were classified as indicated. The percentages of Rab5 endosome per cell are shown with s.d. Control, n=55; ADAP1KD, n=47; ARAP1KD, n=45. Significance was tested using mixed-effects analysis and Sidak's multiple comparison test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns, not significant. Scale bars: 10 µm.