Fig. 2.

Example of Q-Orbitrap-Astral data-independent acquisition. Data-independent acquisition with high-resolution Orbitrap MS¹ scans collected in parallel with narrow-bin, high-resolution Astral MS/MS scans. At a retention time of 22.02 min, scan #230,122, an Orbitrap full scan, was acquired while hundreds of DIA MS/MS were acquired in the Astral mass analyzer. For the m/z range from 650 to 700, 25 DIA scans were acquired over 150 ms resulting in 38 unique peptide identifications. The b- and y-type fragment ions are denoted in blue and red, respectively. Spectra are shown zoomed relative to the base beak due to limited fragmentation of coisolated, singly charged ions. DIA, data-independent acquisition; MS, mass spectrometry; MS/MS, tandem mass spectrometry.