Skip to main content
. 2024 Apr 15;14(7):2656–2674. doi: 10.7150/thno.91456

Figure 4.

Figure 4

99mTc-sdAb20 can specifically target and visualize AXL-expressing cells in the syngeneic murine C1498 AML mouse model. (A) Ex vivo γ-counting of isolated organs from naive and AXLhigh C1498 mice. Organs were isolated 90 min post-injection of radiolabeled (99mTc) sdAb20 or R3B23 control tracer (n=5). (B) Uptake of radiolabeled sdAb20 in various hematological organs of naive and AXLhigh C1498 tumor-bearing immunocompetent mice. Data were represented as organ-to-blood ratio. (C) Flow cytometric analysis of end-stage tumorload (CD45.2+ tumor cells) of different organs (n=8, ± SD). (D) Reconstructed SPECT/CT images of naive and AXLhigh C1498 mice, one hour post-injection of 99mTc-labeled anti-AXL sdAb20 and R3B23 control sdAb (n=8). Images show maximum intensity projections and transversal planes; and are reconstructed with OsiriX software. Representative intensities (%IA/g) are shown next to the images. Dashed circles represent ovaries. %ID/cc = injected dose per cubic centimeter. p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***) and p ≤ 0.0001 (****) were considered statistically significant.