Skip to main content
. 2000 Mar 15;28(6):1424–1427. doi: 10.1093/nar/28.6.1424

Figure 2.

Figure 2

PCNA addition reverses the inhibition of crosslink-induced DNA synthesis by p21. (A) Experiments were performed as described in the legend to Figure 1. PCNA and p21 were incubated together on ice for 15 min followed by addition of HeLa cell extract and a further incubation on ice for 15 min. Other components were then added and the incubation continued for 3 h at 28°C. The addition of PCNA was as follows: lanes 1 and 2, none; lane 3, 0.37 µM; lane 4, 0.75 µM; lane 5, 1.1 µM. (B) Quantitation of the experiment shown in (A). Open circle, DT plasmid; closed circle, CLT plasmid.