Figure 2.

ZMIZ1 is within the ER transcriptional complex. (A) Cells were treated for 24 h with vehicle (ethanol) or with 100 nM fulvestrant (a selective ER degrader) before analysis by PLA. Dual antibody recognition PLA between ER (Ab3575) and either ER (SC8002), IgG or ZMIZ1 demonstrated a significant reduction in both ER and the ER-ZMIZ1 interaction on addition of fulvestrant in ER-positive cell lines, indicating that ER and ZMIZ1 are within the same protein complex. No significant effect on the PLA signal was detected in the ER-IgG negative control or in the ER-negative cell line MDA-MB-231 (Bonferroni adjusted P-value, ^*<0.05, ^**<0.01, ^***<0.001). The error bars indicate s.d. (B) Illustrative images of the ER-ZMIZ1 PLA, taken at 630× magnification. DAPI stained cell nuclei are shown in blue and PLA signals are shown as red dots.