Table 1. The reagents and equipment used in this protocol.

Reagents and equipment	Source	Cat. number
Reagents
NaCl	Sigma-Aldrich	Cat. #S7653
KCl	Sigma-Aldrich	Cat. #P9333
MgCl2	Sigma-Aldrich	Cat. #208337
Sucrose	Sigma-Aldrich	Cat. #S9378
Tricine	Sigma-Aldrich	Cat. #T0377
HEPES	Sigma-Aldrich	Cat. #H3375
Tris	Sigma-Aldrich	Cat. #T1503
Glycerol	Sigma-Aldrich	Cat. #G5516
SDS	Sigma-Aldrich	Cat. #L4390
Bromophenol blue	Sigma-Aldrich	Cat. #B0126
β-ME	Sigma-Aldrich	Cat. #M6250
EDTA	Sigma-Aldrich	Cat. #E1644
Triton X-100	Sigma-Aldrich	Cat. #T8787
Anti-FLAG M2 Affinity beads	Sigma-Aldrich	Cat. #A2220
Protease inhibitor cocktail	Roche	Cat. #5892791001
PMSF	Sigma-Aldrich	Cat. #52332
PBS	Sangon Biotech	Cat. #E607008
OA	Sigma-Aldrich	Cat. #O7501
Equipment
Ultracentrifuge	Beckman	Optima L-100
Centrifuge	Eppendorf	5424R
Nitrogen bomb	Parr instrument	Cat. #4639
Dounce homogenizer	Wheaton	Cat. #357542