| 2× Sample Buffer |
100 mmol/L Tris-HCl (pH 6.8), 4% (m/v) SDS, 20% Glycerol (v/v), 0.04% (m/v) Bromophenol blue, 4% β-ME |
| Buffer A |
20 mmol/L Tricine, 250 mmol/L Sucrose, pH = 7.8 |
| Buffer B |
200 mmol/L HEPES, 100 mmol/L KCl, 2 mmol/L MgCl2, pH = 7.4 |
| RIPA 1 |
50 mmol/L Tris-HCl (pH = 7.5), 150 mmol/L NaCl, 1 mmol/L EDTA, 1% Triton X-100, 10% Glycerol |
| RIPA2 |
50 mmol/L Tris-HCl (pH = 7.5), 300 mmol/L NaCl, 1 mmol/L EDTA, 1% Triton X-100, 10% Glycerol |
| RIPA3 |
50 mmol/L Tris-HCl (pH = 7.5), 500 mmol/L NaCl, 1 mmol/L EDTA, 1% Triton X-100, 10% Glycerol |
