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Authentic hMYH possesses repair activity for 2-OH-A and adenine paired with guanine or 8-oxoG. The RESOURCE S fraction was subjected to nicking assay for various double-stranded oligonucleotides (A). The active fractions were subjected to SDS–PAGE and then the gel was stained with silver (B) or was further analyzed by western blotting, using anti-hMYH which had been pre-adsorbed with TrpE–Sepharose (C) or TrpE-hMYH–Sepharose (D).
