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. 2000 Mar 15;28(6):1355–1364. doi: 10.1093/nar/28.6.1355

Figure 4.

Figure 4

Recombinant hMYH possesses DNA repair activity for 2-OH-A and adenine paired with guanine or 8-oxoG. (A) Western blotting. Extracts (5 µl) prepared from E.coli cells carrying plasmid pXC35:14-3-3 (lane C) or pXC35:hMYHα3-2 (lane Y) were subjected to western blot analysis, using anti-hMYH. (B) DNA repair activities of recombinant hMYH. Various double-stranded oligonucleotides (150 fmol/15 µl reaction) were incubated with extracts (5 µl) prepared from E.coli cells carrying plasmid pXC35:14-3-3 (lane C) or pXC35:hMYHα3-2 (lane Y) for 30 min at 37°C, then 3 µl of 1 N NaOH was added, followed by heating at 95°C for 10 min. Then the mixture was fractionated on an 8% LongRanger gel containing 7 M urea. Relative amounts of cleaved products of each substrate produced by hMYH to that of A:GO substrate are shown.