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. 2000 Mar 15;28(6):1489–1497. doi: 10.1093/nar/28.6.1489

Figure 5.

Figure 5

RNase protection analysis of the alternative splicing pattern of the mouse, rat and human p53 gene in cell lines from different species after transient transfection. (A) Transfection of the murine neuroblastoma N2A cell line with murine p53 (Mp), rat p53 (Rp) or human p53 (Hp) expression vectors. The mouse p53 probe Mp53L was used in lanes 1–4 and the human p53 probe Hp53B was used in lane 5 (see Materials and Methods). C, control (non-transfected cell line); Exo, exogenous (transfected) p53 mRNA; Endo, endogenous p53 mRNA. (B) Transfection of the human Panc and N2A cell lines with the murine p53 expression vector (Mp). The mouse p53 cDNA probe MT7E-E9 is shown schematically. The protected fragments corresponding to the transfected (p53R Exo and p53C Exo) products are indicated. C, control (non-transfected cell line). (C) Transfection of the rat cell lines NBTII (NBT) and NRK with the mouse (Mp) and rat (Rp) p53 expression vectors. RNA in lanes 1 and 2 was protected with the mouse probe Mp53L. RNA in lanes 3 and 4 was protected with the rat p53 probe Rp53L (see Materials and Methods). Additional bands correspond to incomplete RNase digestion products.