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. Author manuscript; available in PMC: 2024 May 20.
Published in final edited form as: ACS Chem Biol. 2020 Dec 4;15(12):3159–3166. doi: 10.1021/acschembio.0c00676

Table 1.

Steady-State Kinetic Parametersa of HsKYNase and PfKYNase for Both KYN and OH-KYN in PBS, pH 7.4 at 37 °C

kynurenine OH-kynurenine
enzyme kcat (s−1) KM (μM) kcat/KM (M−1S1−) kcat (s−1) KM (μM) kcat/KM (M−1S1−)
HsKYNase 0.18 ± 0.02 1200 ± 200 (1.5 ± 0.4) × 102 1.7 ± 0.1 29 ± 5 (6 ± 1.3) × 104
PfKYNase 7 ± 0.35 90 ± 15 (7.7 ± 1.4) × 104 (1.5 ± 0.25) × 103
a

Parameters are shown as the best fit value ± standard error upon fitting the experimental data to either the Michaelis–Menten equation (eq 1 in the SI) or to a linear function (y = ax + b) to calculate kcat/KM.