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. Author manuscript; available in PMC: 2024 May 20.
Published in final edited form as: Sci Immunol. 2023 Dec 8;8(90):eadi3974. doi: 10.1126/sciimmunol.adi3974

Fig. 2. Single-cell cloning of human antigen-specific NK cells.

Fig. 2.

Percentages of antigen-specific lysis by 133 NKCL generated from 19 PLWH against HIV Env, HIV Gag and CMV pp65 (A) after subtracting background (mock-pulsed BLCL) and (B) compared to non-specific killing of mock-pulsed BLCL for each NKCL. Percentages of antigen-specific lysis by 28 NKCL generated from 10 healthy donors against influenza H1N1 NP (C) after subtracting background (mock-pulsed BLCL) and (D) compared to non-specific killing of mock-pulsed BLCL for each NKCL. Full circle, positive for IgG antibodies against influenza A. Empty circle, not tested for the presence of IgG antibodies against influenza A. CAM cytotoxicity assays were used to evaluate lysis after co-culture of NKCL with autologous BLCL pulsed with indicated peptide pools. Non-specific lysis was assessed by measuring killing of mock-pulsed autologous BLCL, self-peptides-pulsed BLCL (negative control) and HLA-E-deficient K562 cells (positive control). (E) Heatmap of antigen-specific lysis after subtracting background for 16 NKCL from 8 PLWH with the highest specific killing against HIV Gag and/or ENV. NKCL generated from the same PLWH are displayed in a unique color. NKCL displaying over 15% specific killing against both HIV Env and HIV Gag or HIV Env and CMV pp65 are indicated in bold font. Statistical significance was tested using Wilcoxon signed-rank test (B and D). ns, not significant.