Table 2. Agreement in species identification by miLab (A) or microscopy (B) and qPCR.
A) miLab | qPCR (all densities) | ||||
Total | Pf | Pv | Pf+Pv | Negative | |
miLab negative | 370 | 146 | 33 | 54 | 137 |
miLab detects Pf | 209 | 125 | 13 | 48 | 23 |
miLab detects Pv | 75 | 8 | 33 | 30 | 4 |
miLab detects Pf+Pv | 5 | 0 | 0 | 5 | 0 |
Total | 659 | 279 | 79 | 137 | 164 |
qPCR (>20 parasites/μL) | |||||
Total | Pf (>20 parasites/μL) | Pv (>20 parasites/μL) | Pf+Pv (>20 parasites/μL) | Negative | |
miLab negative | 11 | 6 | 5 | 0 | NA |
miLab detects Pf | 144 | 130 | 9 | 5 | NA |
miLab detects Pv | 64 | 6 | 50 | 8 | NA |
miLab detects Pf+Pv | 5 | 0 | 0 | 5 | NA |
Total | 224 | 142 | 64 | 18 | NA |
B) Microscopy | qPCR (all densities) | ||||
Total | Pf | Pv | Pf+Pv | Negative | |
Microscopy negative | 448 | 181 | 45 | 64 | 158 |
Microscopy detects Pf | 131 | 83 | 7 | 40 | 1 |
Microscopy detects Pv | 68 | 12 | 25 | 26 | 5 |
Microscopy detects Pf+Pv | 12 | 3 | 2 | 7 | 0 |
Total | 659 | 279 | 79 | 137 | 164 |
qPCR (>20 parasites/μL) | |||||
Total | Pf (>20 parasites/μL) | Pv (>20 parasites/μL) | Pf+Pv (>20 parasites/μL) | Negative | |
Microscopy negative | 29 | 18 | 10 | 1 | NA |
Microscopy detects Pf | 128 | 112 | 11 | 5 | NA |
Microscopy detects Pv | 57 | 9 | 41 | 7 | NA |
Microscopy detects Pf+Pv | 10 | 3 | 2 | 5 | NA |
Total | 224 | 142 | 64 | 18 | NA |
In the top panels, infections positive by qPCR irrespective of density are included. In the bottom panels infections at densities >20 parasites/μL by qPCR are included. For the estimate for P. falciparum, infections at densities >20 P. falciparum parasites/μL by qPCR and <20 P. vivax parasites/μL are included, and vice-versa for the estimate for P. vivax.