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. 2024 May 20;4(5):e0003091. doi: 10.1371/journal.pgph.0003091

Table 2. Agreement in species identification by miLab (A) or microscopy (B) and qPCR.

A) miLab qPCR (all densities)
Total Pf Pv Pf+Pv Negative
miLab negative 370 146 33 54 137
miLab detects Pf 209 125 13 48 23
miLab detects Pv 75 8 33 30 4
miLab detects Pf+Pv 5 0 0 5 0
Total 659 279 79 137 164
qPCR (>20 parasites/μL)
Total Pf (>20 parasites/μL) Pv (>20 parasites/μL) Pf+Pv (>20 parasites/μL) Negative
miLab negative 11 6 5 0 NA
miLab detects Pf 144 130 9 5 NA
miLab detects Pv 64 6 50 8 NA
miLab detects Pf+Pv 5 0 0 5 NA
Total 224 142 64 18 NA
B) Microscopy qPCR (all densities)
Total Pf Pv Pf+Pv Negative
Microscopy negative 448 181 45 64 158
Microscopy detects Pf 131 83 7 40 1
Microscopy detects Pv 68 12 25 26 5
Microscopy detects Pf+Pv 12 3 2 7 0
Total 659 279 79 137 164
qPCR (>20 parasites/μL)
Total Pf (>20 parasites/μL) Pv (>20 parasites/μL) Pf+Pv (>20 parasites/μL) Negative
Microscopy negative 29 18 10 1 NA
Microscopy detects Pf 128 112 11 5 NA
Microscopy detects Pv 57 9 41 7 NA
Microscopy detects Pf+Pv 10 3 2 5 NA
Total 224 142 64 18 NA

In the top panels, infections positive by qPCR irrespective of density are included. In the bottom panels infections at densities >20 parasites/μL by qPCR are included. For the estimate for P. falciparum, infections at densities >20 P. falciparum parasites/μL by qPCR and <20 P. vivax parasites/μL are included, and vice-versa for the estimate for P. vivax.