Fig. 1.

NPY and NPY derivates binding to the Y₁R. a Amino acid sequences of porcine NPY, G³⁴-NPY, F⁷,P³⁴-NPY and K¹⁸-PEG_20K-F⁷,P³⁴-NPY with altered amino acids in bold letters. b Model of NPY bound to the Y₁R,

modified from Yang et al. [21]. Positions 7, 18 and 34 used for modification are highlighted in circles. c Binding of peptides to the Y₁R was measured by competition binding experiments with ¹²⁵I-PYY (75 pM) of membrane preparations of stably transfected HEK293-Y₁R cells and is displayed as mean ± SEM of three independent experiments performed in technical triplicate