Fig. 6.

The kinetic BRET profile of the Y₁R-G_o complex contains an association component. a The limited experimentally observed net dissociation can be explained by an additional re-association component of G_o to available receptors. The observed net dissociation accordingly corresponds to receptors that are not available by internalization. b In line with this hypothesis, a slower apparent dissociation is observed when higher amounts of Gα_o-Venus are expressed, which would accelerate k_on. c After agonist washout the signal approaches the baseline only very slowly, likely due to re-association of G_o to recycled Y₁ receptors. Shown is a buffer-corrected representative example of n = 3 independent experiments conducted in technical quadruplicate