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. 2000 Mar 15;28(6):1428–1438. doi: 10.1093/nar/28.6.1428

Figure 2.

Figure 2

Deletion of a 10 nt fragment in the 3′-UTR of I-PpoI ORF affects splicing of the intron. (A) The sequence of the UTR1 is shown. The numbers underneath indicate regions that are deleted in the five serial deletion mutants. The boxed nucleotides are deleted in the plasmid pNOY103I3Δ10. (B) Northern blot of the 10 nt deletion mutant. PpLSU3 or its mutant forms were cloned into the intron insertion site in the 35S rDNA unit of the plasmid pNOY103 (34,35). RNA was prepared from yeast strain NOY505R transformed and expressing each of the three plasmids. Ten micrograms of total RNA were loaded for northern blot analysis. WT, wild-type PpLSU3; MP7, the G-binding site of PpLSU3 mutated; Δ10, the fifth 10 nt of PpLSU3 deleted. The arrows point to pre-rRNA species containing the unspliced intron.