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. 2000 Mar 15;28(6):1428–1438. doi: 10.1093/nar/28.6.1428

Figure 3.

Figure 3

Expression of functional α-fragment from PpLSU3-α integrated into rDNA. (A) Constructs and strains used. (B) β-gal activity assay. I, pYESMlacZ transformed into strain NOY505; II, plasmid RS404GalΩT integrated into the trp1 locus of strain NOY505 to generate strain NOY505GalΩT; III, pYESMα transformed into NOY505; IV, pYESMα transformed into strain NOY505GalΩT; V, PpLSU3-α integrated into strain NOY505GalΩT to create strain NOY505GalΩT/JLα. (C) Northern blot analysis of the α-integrated strain. 1, 10 µg of total RNA from NOY505GalΩT transformed with pYESMα; 2, 10 µg of total RNA from strain NOY505GalΩT/JLα; 3, 1 µg of total RNA from strain INVSc2/I3; 4, 1 µg of total RNA from NOY505GalΩT/JLα.