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Schematic drawing of the randomization approach. (A) Construction of the randomized plasmid pool. The boxes and shadings representing exon, intron and lacZ sequences are the same as in Figure 3. The randomized region is indicated as NNN. The drawing is not to scale. Restriction sites: B, Bsu36I; S, SphI. (B) Identification of sequences that allow integration into rDNA repeats.
