Table 1. Yeast strains used in this study.
| Strain | Genotype | Description | Reference |
|---|---|---|---|
| 1. NOY505 | MATa ade2 ura3 leu2 trp1 his3 can1 | 51 | |
| 2. INVSc2/I3 | MATα his3-Δ200 ura3-167 25S::I3 | PpLSU3 integrated | 37 |
| 3. NOY505R | MATa ade2 ura3 leu2 trp1 his3 can1 | Insertion of T in I-PpoI target site | This study |
| 25S::1925T | |||
| 4. NOY505GalΩT | MATa ade2 ura3 leu2 trp1 his3 | Plasmid RS404GalΩT integrated into trp1 locus | This study |
| can trp1::RS404GalΩT | |||
| 5. NOY505GalΩT/Jlα | MATa ade2 ura3 leu2 trp1 his3 | JLI3α integrated into rDNA of NOY505GalΩT | This study |
| can trp1::RS404GalΩT 25S::I3α | |||
| 6. NOY505GalΩT/JlαΔ7 | MATa ade2 ura3 leu2 trp1 his3 | JLI3αΔ7 integrated, similar to #4 | This study |
| can trp1::RS404GalΩT 25S::I3αΔ7 |
NOY505GalΩT/JlαΔ8, NOY505GalΩT/JlαΔ9, NOY505GalΩT/JlαΔ10, NOY505GalΩT/JlαΔ1 and NOY505GalΩT/JlαΔ11 are identical to #6, except with the slightly longer deletion, as shown in Figure 5.