Fig. 5.

Therapeutic effect of B2M^–UMSCs and exosomes is mediated by miR-24/Bim. a Heatmap of miRNA sequencing data from UMSCs, UMSC-exosomes, B2M^–UMSCs and B2M^–UMSCs-exosome (green, downregulated; red, upregulated). b RT-qPCR analysis of miR-24 expression in cells and their derived exosomes. c Real-time PCR assessment of miR-24 expression in different tissues of rats. d In situ hybridization using a miR-24 locked nucleic acid probe in B2M^–UMSCs (scale bar, 20 μm). e Bioinformatics analysis identified Bim as a putative target of miR-24. f TargetScan prediction of conserved miR-24 binding sites in the Bim 3′ UTR of human and rat. Base pairs highlighted in color (miR-24 in red and Bim 3′ UTR in green) are seed sequences complimentary between miRNA and target. g Luciferase assays of HEK293T cells co-transfected with psiCHECK™^-2 vector-3′ UTR and LV3-miR-24 vector for 48 h (n = 3; **p < 0.01). h Expression of miR-24 and Bim mRNA in the infarct zone (IZ) and distant zone (DZ) of the rat myocardium at 1 and 3 days after MI (n = 3; *p < 0.05). i Western blots of Bim expression in the IZ and DZ 3 days after MI