Fig. 1.

Replisome is disengaged from nascent DNA upon acute HU treatment in hTERT-RPE cells. a The protein ID of the replisome components and the DNA repair-related proteins identified in the iPOND-MS experiment and their normalized relative abundance in each condition are represented. b Cells were treated and harvested as in a. The proteins present in iPOND extracts were analyzed by WB with the indicated antibodies. Input: nuclear extract. Histone 3 (H3) was used as an immunoprecipitation control. l.e. long exposure, s.e. short exposure, (−) negative control (no EdU), pulse 15 min EdU, chase pulse followed by 2 h with low-dose thymidine. For experimental setting detail, see also Fig. S1 and Tables S1, S2, and S3