Fig. 3.

hTERT-RPE cells maintain the competence to restart upon acute replication stress in the absence of CDK activity. a, b Asynchronously growing cells were labelled and treated as indicated. DNA fibers were prepared and stained. The percentage of stalled, restarted, and new origin firing events is shown. Around 1500 fibers were counted in each condition. Error bars represent standard deviation (paired t test, n = 3). Representative images are shown (bottom-right panel). Cdc7i: Cdc7 inhibitor. See also Fig. S3