Fig. 4.

The variants of p210 BCR–ABL used in the study. Schematic representation of p190 and p210 BCR–ABL. All truncated or point-mutated p210 BCR–ABL constructs contain N-terminal FLAG epitope. The amino acid substitutions are indicated in red; CC coiled-coil domain, DH double homology domain, PH pleckstrin homology domain, SH3, SH2 Src homology domain, TK tyrosine kinase domain, IDR intrinsically disordered region, FABD F-actin binding domain, NLS nuclear localization signal, PR proline-rich region. The numbering of BCR–ABL domains follows the total protein length (2030 aa in p210), numbering of individual residues is relative to the position of given amino acid in the individual sequence of BCR or cABL1b, with the exception of K271H, which is based on cABL1a