Fig. 5.

NPHP5-mediated BF assembly is cell-type specific. RPE-1, ARPE-19, HK-2, HeLa, U2OS, PC-3, MCF-7, DU-145, and SAOS-2 cells transfected with NS (non-specific) or NPHP5 siRNAs and grown in the a absence or c presence of serum were stained with the indicated antibodies. Scale bar, 1 µm. b, d Fluorescence intensity of ninein at the centrosome was quantitated and set to 100% in NS siRNA-transfected cells. For quantitation, at least 20 cells for each condition were analyzed, and the mean and standard error of three independent experiments are presented