Fig. 7.

BF loss due to ablation of NPHP5, Kif3a or ODF2 can be rescued. a Quiescent RPE-1 cells transfected with NS (non-specific) or the indicated siRNAs and plasmid expressing the indicated protein were stained with the indicated antibodies and with DAPI (blue). Scale bar, 2 µm. b Fluorescence intensity of ninein at the centrosome was quantitated and set to 100% in NS siRNA-transfected cells. For quantitation, at least 20 cells for each condition were analyzed, and the mean and standard error of three independent experiments are presented. c Quiescent RPE-1 cells transfected with the indicated siRNAs were stained with the indicated antibodies and with DAPI (blue). Scale bar, 2 µm. d Fluorescence intensity of ninein at the centrosome was quantitated and set to 100% in NS siRNA-transfected cells. For quantitation, at least 20 cells for each condition were analyzed, and the mean and standard error of three independent experiments are presented. e Schematic model of BF assembly