Fig. 8.

Cilia loss due to ablation of NPHP5, Kif3a or ODF2 can be rescued. a Quiescent RPE-1 cells transfected with NS (non-specific) or the indicated siRNAs and plasmid expressing the indicated protein were stained with the indicated antibodies and with DAPI (blue). Scale bar, 2 µm. b The percentage of GFP positive cells with cilia was scored. At least 100 cells for each condition were analyzed, and the mean and standard error of three independent experiments are presented. c Quiescent RPE-1 cells transfected with the indicated siRNAs were stained with the indicated antibodies and with DAPI (blue). Scale bar, 2 µm. d The percentage of cells with cilia was scored. At least 100 cells for each condition were analyzed, and the mean and standard error of three independent experiments are presented