Fig. 2.

The AQP2-L137P mutant exists as an immature high-mannose form when expressed in kidney epithelial cells. a Cell lysates isolated from HEK293 cells transiently transfected with AQP2-WT and AQP2-L137P constructs were deglycosylated using PNGase F or EndoH and immunoblotted for AQP2. PNGase treatment shifted higher molecular weight bands of AQP2-WT and AQP2-L137P to a similar size, whereas EndoH only shifted the higher molecular weight bands of AQP2-L137P indicating it is a high-mannose form. b Immunblotting of PNGase-treated cell lysates from MDCK cells stably expressing AQP2-WT or AQP2-L137P revealed a similar size shift in the mutant cells. Each lane represents a sample obtained from a different batch of cells