Table 1.
Identification of proteins commonly associated with mitochondria found from EGFP to EI24 affinity purification in HEK293T cells
| Protein ID | Genes | Description | Peptide no. |
|---|---|---|---|
| P38646 | HSPA9 | GRP75_HUMAN Stress-70 protein, mitochondrial | 26 |
| P21796 | VDAC1 | VDAC1_HUMAN Voltage-dependent anion-selective channel protein 1 | 1 |
| P05141 | SLC25A5 | ADT2_HUMAN ADP/ATP translocase 2 | 4 |
| P40939 | HADHA | ECHA_HUMAN Trifunctional enzyme subunit alpha, mitochondrial | 27 |
| P12236 | SLC25A6 | ADT3_HUMAN ADP/ATP translocase 3 | 2 |
| Q00325;F8VVM2 | SLC25A3 | MPCP_HUMAN Phosphate carrier protein, mitochondrial | 2 |
| Q8TC07 | TBC1D15 | TBC15_HUMAN TBC1 domain family member 15 | 13 |
| P27348 | YWHAQ | 1433T_HUMAN 14-3-3 protein theta | 3 |
| P11021 | HSPA5 | GRP78_HUMAN 78 kDa glucose-regulated protein | 9 |
| P61981 | YWHAG | 1433G_HUMAN 14-3-3 protein gamma | 7 |
| P0DMV9;P0DMV8;A0A0G2JIW1 | HSPA1B;HSPA1A;HSPA1B | A0A0G2JIW1_HUMAN Heat shock 70 kDa protein 1B | 11 |
| Q9Y277 | VDAC3 | VDAC3_HUMAN Voltage-dependent anion-selective channel protein 3 | 4 |
| Q04917 | YWHAH | 1433F_HUMAN 14-3-3 protein eta | 3 |
| P31946 | YWHAB | 1433B_HUMAN 14-3-3 protein beta/alpha | 2 |
| P53007 | SLC25A1 | TXTP_HUMAN Tricarboxylate transport protein, mitochondrial | 4 |
| Q02978 | SLC25A11 | M2OM_HUMAN Mitochondrial 2-oxoglutarate/malate carrier protein | 2 |
| P35232 | PHB | PHB_HUMAN Prohibitin | 16 |
| Q9BQT8 | SLC25A21 | ODC_HUMAN Mitochondrial 2-oxodicarboxylate carrier | 2 |
| H3BPZ1;H3BS72;Q9P035 | HACD3 | H3BS72_HUMAN Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase | 6 |
| Q9UBX3;F6RGN5 | SLC25A10 | F6RGN5_HUMAN Mitochondrial dicarboxylate carrier | 3 |
| B0QYW5;F6RTR7;O43808 | SLC25A17 | PM34_HUMAN Peroxisomal membrane protein PMP34 | 2 |
| P38606 | ATP6V1A | VATA_HUMAN V-type proton ATPase catalytic subunit A | 4 |
| O43819 | SCO2 | SCO2_HUMAN Protein SCO2 homolog, mitochondrial | 3 |
| Q9H3P7 | ACBD3 | GCP60_HUMAN Golgi resident protein GCP60 | 2 |
| P45880 | VDAC2 | VDAC2_HUMAN Voltage-dependent anion-selective channel protein 2 | 4 |
| J3QTB2;Q9BRQ6;J3QTA6 | CHCHD6 | J3QTA6_HUMAN MICOS complex subunit | 1 |
| P08238 | HSP90AB1 | HS90B_HUMAN Heat shock protein HSP 90-beta | 3 |
| Q15046 | KARS | SYK_HUMAN Lysine–tRNA ligase | 3 |
| Q9UJS0 | SLC25A13 | CMC2_HUMAN Calcium-binding mitochondrial carrier protein Aralar2 | 4 |
| P21964 | COMT | COMT_HUMAN Catechol O-methyltransferase | 1 |
| P28331;B4DJ81 | NDUFS1 | NDUS1_HUMAN NADH–ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | 4 |
| O95573 | ACSL3 | ACSL3_HUMAN Long-chain-fatty-acid–CoA ligase 3 | 4 |
| A0A0D9SF53;A0A0D9SFB3;A0A0D9SG12;O00571 | DDX3X | A0A0D9SF53_HUMAN ATP-dependent RNA helicase DDX3X | 4 |
| A8MX94;P09211 | GSTP1 | GSTP1_HUMAN Glutathione S-transferase P | 2 |
| P10809 | HSPD1 | CH60_HUMAN 60 kDa heat shock protein, mitochondrial | 4 |
| P49327 | FASN | FAS_HUMAN Fatty-acid synthase | 21 |
| O75746 | SLC25A12 | CMC1_HUMAN Calcium-binding mitochondrial carrier protein Aralar1 | 3 |
| P12277 | CKB | KCRB_HUMAN Creatine kinase B-type | 1 |
| Q7L8L6 | FASTKD5 | FAKD5_HUMAN FAST kinase domain-containing protein 5, mitochondrial | 4 |
| Q7Z7H8 | MRPL10 | RM10_HUMAN 39S ribosomal protein L10, mitochondrial | 1 |
| P25705 | ATP5A1 | ATPA_HUMAN ATP synthase subunit alpha, mitochondrial | 2 |
| P63104 | YWHAZ | 1433Z_HUMAN 14-3-3 protein zeta/delta | 5 |
| C9IY40;Q5T653 | MRPL2 | RM02_HUMAN 39S ribosomal protein L2, mitochondrial | 1 |
| P42704 | LRPPRC | LPPRC_HUMAN Leucine-rich PPR motif-containing protein, mitochondrial | 8 |
| Q5T3Q7;Q9H583 | HEATR1 | HEAT1_HUMAN HEAT repeat-containing protein 1 | 13 |
| P42345 | MTOR | MTOR_HUMAN Serine/threonine–protein kinase mTOR | 12 |
| P04049 | RAF1 | RAF1_HUMAN RAF proto-oncogene serine/threonine–protein kinase | 2 |
| B4DNK4;P14618 | PKM | KPYM_HUMAN Pyruvate kinase PKM | 2 |
| Q7L4E1 | MIGA2 | MIGA2_HUMAN Mitoguardin 2 | 2 |
| Q92538 | GBF1 | GBF1_HUMAN Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 | 6 |
| P16435 | POR | NCPR_HUMAN NADPH–cytochrome P450 reductase | 2 |
| P33121;B7Z3Z9;D6RER0;E7EPM6 | ACSL1 | ACSL1_HUMAN Long-chain-fatty-acid–CoA ligase 1 | 1 |
| P43304 | GPD2 | GPDM_HUMAN Glycerol-3-phosphate dehydrogenase, mitochondrial | 1 |
| A0A140T9T7;Q03518 | TAP1 | A0A140T9T7_HUMAN Antigen peptide transporter 1 | 2 |