Fig. 2.

ERK1/2 inhibition prevents IR-induced downregulation of PGC-1α and downstream targets and attenuates kidney dysfunction. A Representative immunoblot of PGC-1α, NDUFS1, NDUFB8, COX1, pERK1/2, and tERK1/2 following IR AKI. p phosphorylated, t total. B Densitometry analysis of PGC-1α, NDUFS1, NDUFB8, and COX1 proteins following 24 h IR. C mRNA expression of PGC-1α, TFAM, SOD2, and FOXO1 following IR AKI. D Serum creatinine was assessed at 24 h after IR AKI. Data are represented as mean ± S.E.M., n ≥ 4. Different superscripts indicate statistically significant differences (P < 0.05); ‘a’ means not statistically different from sham, ‘b’ means statistically different from sham, ‘c’ means statistically different from sham and IR