Fig. 6.

ERK1/2 physiologically regulates miR34a and ERK1/2 inhibition decreases miR-34a, leading to increases in NAMPT Protein. A Representative immunoblot of pERK1/2, tERK1/2, and NAMPT protein 4 h after trametinib administration. p phosphorylated, t total. B Densitometry analysis of NAMPT protein, and NAMPT mRNA and miR34a expression measured at 4 h after trametinib treatment. C NAD + and NADH content in renal cortex measured at 4 h after trametinib treatment. D miR34a expression after 34a mimic treatment with and without trametinib in mice. E Representative immunoblot of NAMPT protein after 34a mimic treatment in the renal cortex. F Densitometry analysis of NAMPT protein after 34a mimic treatment. Data are represented as mean ± S.E.M., n ≥ 5. Different superscripts indicate statistically significant differences (P < 0.05); ‘a’ means not statistically different from sham, ‘b’ means statistically different from sham. c’ means statistically different from IR