Fig. 7.

Glutamate-induced NO release in hCMEC/D3 cells through the stimulation of mGluR1 and mGluR5. a Glutamate (100 µM) caused a robust increase in DAF/FM fluorescence in hCMEC/D3 cells, that was strongly reduced by either L-NAME (100 µM, 2 h), an inhibitor of NO synthase, or BAPTA (30 µM, 2 h), a membrane-permeable intracellular Ca²⁺ chelator. b Glutamate induced a massive increase in NO production, which was inhibited by MCPG (150 µM, 20 min). cTrans-ACPD (100 µM) induced robust NO release in hCMEC/D3 cells, thereby phenocopying the response to glutamate. d Glutamate-induced NO release was reduced by CPCCOEt (100 µM, 10 min) and MTEP (100 µM, 10 min). e Bar histogram shows the mean ± SE of the percentage of responding cells under the designated treatments. The asterisk indicates p < 0.05 as compared to control cells. f Bar histogram shows the mean ± SE of the amplitude of the response under the designated treatments. The asterisk indicates that p < 0.05 as compared to control cells