Fig. 8.

Glutamate-induced intracellular Ca²⁺ signaling drives NO release in hCMEC/D3 cells. a Glutamate-induced NO release was not prevented by removal of extracellular Ca²⁺ (0Ca²⁺). Glutamate was administered at 100 μM. b Glutamate-induced NO release was not prevented by SOCE inhibition with Pyr6 (10 µM, 10 min). Glutamate was administered at 100 μM. c Glutamate-induced NO release was abolished by U73122 (10 µM, 30 min) and by 2-APB (50 µM, 30 min). Glutamate was administered at 100 μM. d Glutamate-induced NO release was inhibited by GPN (200 μM) and NED-19 (100 μM, 30 min). Glutamate was administered at 100 μM. e Bar histogram shows the mean ± SE of the percentage of responding cells under the designated treatments. The asterisk indicates p < 0.05. f Bar histogram shows the mean ± SE of the amplitude of the response under the designated treatments. The asterisk indicates that p < 0.05. NR no response