Fig. 7.

Igf3 expression is regulated by androgen. a, b The transcript level of igf3 in testicular explants incubated with increasing concentrations of MT and 11-KT ex vivo for 4 days. Data are expressed as the mean ± SD. Different letters indicate statistical differences at p < 0.05 as determined by one-way ANOVA, followed by Tukey’s/Kramer post hoc test. c Treatment with the androgen synthesis inhibitor trilostane significantly down-regulated igf3 mRNA expression. d, e, f Real-time PCR and IF results showed that igf3 expression was significantly down-regulated in the cyp11c1^−/− testes compared with that in the control testes. g, h IF analysis showed that cyp11c1 expression was not influenced in the igf3^−/− testes. i Knockouts of igf3 in the XY fish resulted in elevated serum 11-KT concentrations compared with the control fish. Data are expressed as the mean ± SD of triplicates. The difference between two groups was statistically examined with unpaired two tailed Student’s t test; *p < 0.05; **p < 0.01; ***p < 0.001. Scale bar 15 μm (d), 50 μm (f)