Table 2.
Primary and secondary antibodies used for immunohistochemistry
| Tissue species | Immunohistochemical method | Antibody | Reference | Dilution |
|---|---|---|---|---|
| Rat | Chromogen-based simple immunohistochemistry | Rb anti-Fz1 | ab71342, Abcam | 1:250 |
| Rb anti-Iba1 | 019-19741, Wako | 1:1000 | ||
| Mo anti-GFAP | G3893, Sigma-Aldrich | 1:1000 | ||
| Rb anti-NG2 | AB5320, Millipore | 1:250 | ||
| Biotinilated Go anti-Rb | BA-1000, Vector | 1:500 | ||
| Biotinilated Ho anti-Mo | BA-2001, Vector | 1:500 | ||
| Fluorescence-based simple immunohistochemistry | Mo anti-APC | OP80, Calbiochem | 1:100 | |
| Rb anti-NeuN | MABN140, Millipore | 1:100 | ||
| Rb anti-serotonin | S5545, Sigma-Aldrich | 1:500 | ||
| Dylight 594-linked Go anti-Rb | ab96897, Abcam | 1:500 | ||
| Dylight 594-linked Go anti-Mo | ab96881, Abcam | 1:500 | ||
| Fluorescence-based double immunohistochemistry | Rb anti-Fz1 | ab71342, Abcam | 1:50 | |
| Mo anti-GFAP | G3893, Sigma-Aldrich | 1:1000 | ||
| Mo anti-NeuN | MABN140, Millipore | 1:100 | ||
| Mo anti-APC | OP80, Calbiochem | 1:100 | ||
| Mo anti-NF200 | N0142, Sigma-Aldrich | 1:2000 | ||
| Mo anti-OX42 | ab58457, Abcam | 1:500 | ||
| Mo anti-NG2 | 37-2700, Zymed | 1:100 | ||
| Mo anti-RECA1 | MCA970GA, AbD Serotec | 1:250 | ||
| Dylight 594-linked Go anti-Rb | ab96897, Abcam | 1:500 | ||
| Dylight 594-linked Go anti-Mo | ab96881, Abcam | 1:500 | ||
| Human | Chromogen-based simple immunohistochemistry | Rb anti-Fz1 | ab71342, Abcam | 1:500 |
| Rb anti-Fz2 | ab94913, Abcam | 1:500 | ||
| Rb anti-Fz3 | ab188974, Abcam | 1:50 | ||
| Rb anti-Fz4 | ab83042, Abcam | 1:1000 | ||
| Rb anti-Fz5 | ab75234, Abcam | 1:500 | ||
| Rb anti-Fz6 | ab150545, Abcam | 1:100 | ||
| Rb anti-Fz7 | ab64636, Abcam | 1:1000 | ||
| Rb anti-Fz8 | ab75235, Abcam | 1:50 | ||
| Rb anti-Fz9 | ab61430, Abcam | 1:50 | ||
| Rb anti-Fz10 | ab83044, Abcam | 1:500 | ||
| Rb anti-Ryk | AP7677a, Abgent | 1:500 | ||
| Rb anti-Ror1 | AP7671d, Abgent | 1:50 | ||
| Rb anti-Ror2 | AP7672d, Abgent | 1:50 | ||
| Mo anti-PTK7 | 400005754-M06, Abnova | 1:500 | ||
| Rb anti-pLRP6 (Ser 1490) | 2568L, Cell Signaling | 1:100 | ||
| Biotinilated Go anti-Rb | BA-1000, Vector | 1:500 | ||
| Biotinilated Ho anti-Mo | BA-2001, Vector | 1:500 | ||
| Fluorescence-based double immunohistochemistry | Rabbit anti-Fz1 | ab71342, Abcam | 1:100 | |
| Mo anti-GFAP | G3893, Sigma-Aldrich | 1:1000 | ||
| Mo anti-CNPase | SMI-91R, Covance | 1:100 | ||
| Mo anti-CD31 | #3528, Cell Signaling | 1:100 | ||
| Mo Pan Neuronal Marker | MAB2300, Millipore | 1:50 | ||
| Go anti-Iba1 | ab5076, Abcam | 1:100 | ||
| Dylight 594-linked Go anti-Rb | DI-1594, Vector | 1:500 | ||
| Dylight 488-linked Ho anti-Mo | DI-2488, Vector | 1:500 | ||
| Alexa 488-linked Do anti-Go | A11055, Invitrogen | 1:1000 |