Fig. 6.

KDM4A controlled lineage commitment by demethylating H3K9me3 on Sfrp4 and C/ebpα promoters. The mRNA (a, c) and protein (b) levels of C/EBPα and SFRP4 were examined after Kdm4a overexpression (a, b) or ML324 treatment (c). The protein levels of the major components of canonical Wnt signaling were examined following Kdm4a overexpression (d). KDM4A occupancy on the promoters of C/ebpα and Sfrp4 was examined using ChIP assay (e, f). Binding of H3K9me3 to the promoters of C/ebpα and Sfrp4 was examined using ChIP assay (g, h). Values are mean ± SD (n = 3). *P < 0.05 vs. vector