Fig. 2.

Structural characterization of the CFYWH- and FYWH-depleted proteins found in PDB: a NMR solution structure of a CFYWH-depleted 10–48 deletion mutant (residues 1–9/49–123) of the transcarboxylase biotin carrier subunit (also known as biotin carboxyl carrier protein, BCCP) from Propionibacterium freudenreichii subsp. shermanii (PDB ID: 1O78); b crystal structure of the homohexameric molybdenum–pterin-binding protein 2 (molbindin-2 or MopII) from Clostridium pasteurianum (PDB ID: 1GUT); c aligned structures of the molbindin-2 protomers. Structures were aligned using a MultiProt server [127]. Plot was created using VMD platform [128]; d high-resolution cryo-electron microscopy structure of a 40S ribosomal protein S33 from Trypanosoma brucei brucei (strain 927/4 GUTat10.1). Structure of this protein was extracted from of the cryo-EM structure of bacterial ribosome (PDB ID: 4V8M-AZ). Plot was created using VMD platform [128]; e solution NMR structure of a 30S bacterial ribosomal protein S28E from Methanobacterium thermoautotrophicum (PDB ID: 1NE3); f aligned structures of a eukaryotic 40S ribosomal protein rpS28e from Tetrahymena thermophila (PDB ID: 4V5O-A1/B1 and PDB ID: 4BTS-A1/B1/C1/D1). Corresponding structures were extracted from the crystal structures of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1 (PDB ID: 4V5O-A1/B1) and the crystal structure of the eukaryotic 40S ribosomal subunit in complex with eIF1 and eIF1A (PDB ID: 4BTS-A1/B1/C1/D1). Structures were aligned using a MultiProt server [127]. Plot was created using VMD platform [128]; g solution NMR structure of the pulmonary surfactant-associated polypeptide C (SP-C) solved in apolar solvent [a mixed solvent of C₂H₃Cl/C₂H₃OH/1 M HCl 32:64:5 (v/v)] (PDB ID: 1SPF); and h crystal structure of the metal-bound dimer rat metallothionein-2 (PDB ID: 4MT2)