Fig. 1.

Pathogenic bacteria or bacteria-conditioned medium induce 5-LOX translocation in non-immunocompetent HEK293 cells. Subcellular localization of 5-LOX was determined by immunofluorescence microscopy in HEK293 cells stably expressing human 5-LOX (stained in red) and FLAP (green). a HEK_5-LOX/FLAP cells were incubated with intact pathogenic E. coli (O6:K2:H1), S. aureus (LS1), non-pathogenic E. coli BL21 (DE3), or S. carnosus (TM300) using an MOI of 1:50 (HEK293 cells vs. bacteria) for indicated time periods. b Bacteria-conditioned medium (BCM) was collected by sterile filtration of overnight cultures of E. coli [O6:K2:H1 or BL21 (DE3)], S. aureus (LS1), and S. carnosus (TM300) followed by stimulation of HEK_5-LOX/FLAP cells in PBS^+/+ with 10% of BCM. Microscopic images are given as overlay of 5-LOX and FLAP staining. Results shown are representative for at least three independent experiments