Fig. 6.

CX3CR1 is critical for the humoral immune response elicited by Schistosoma japonicum Infection. 6–8 weeks old C57BL/6 WT and CX3CR1 KO mice were percutaneously infected with 25 cercariae of Schistosoma japonicum and splenic B cells were collected after 5 weeks. Isolated splenic B cells were stained for T1, T2 and FO (a), MZ (e), GC (g), PC and PBC (i), B cells switched and unswitched (l) and assessed using flow cytometry. Shown are representative flow cytometry plots from three independent experiments and the summary statistics of percentage (± SEM) and absolute numbers of extracted splenic B cells subsets were shown (b–d, f, h, j, k, m and n). Hematoxylin and eosin staining of spleen from WT and CX3CR1 KO mice (o). The GC areas in the spleen of CX3CR1 KO mice by HE staining (p) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001