Fig. 2.

Phenotype characterization of MODY 2 in F1 GCK-NFS rabbits. a Sequences of mutant GCK gene in F1 rabbits. PAM sites are underlined and highlighted in red; target sequences are green; deletions (−) are shown. WT wild type. b–g Survival curves of wild-type (WT) rabbits and rabbits with GCK heterozygous mutation (GCK-Het) and homozygous mutation (GCK-Hom) in the F1 generation (b), fasting blood glucose (c), Intraperitoneal glucose tolerance test result (d), blood pro-insulin C peptide level (e) and fructosamine (f) levels are shown. g Relative mRNA levels of of GCK gene were determined by qRT-PCR in the pancreas and liver of WT and mutant rabbits and are shown. The data were analyzed by t tests using the Graphpad Prism software. h Analysis of glucokinase content in pancreas and liver of WT and mutation rabbits. The data were expressed as the mean ± SEM using GraphPad Prism software. A probability of p < 0.05 was considered as statistically significant. *, p < 0.05; **, p < 0.01; ***, p < 0.005. ns not significant