Fig. 5.

Pathological observation of multiple organs in F1 mutation rabbits. a Superficial ulceration on forepaw and hindpaws of a homozygous GCK-NFS rabbit (GCK-Hom) and wild-type (WT) rabbits are shown. b Histological images of skeletal muscle and myocardium sections of a WT and a homozygous GCK-NFS (GCK-Hom) rabbit with hematoxylin–eosin staining (HE), periodic acid-Schiff staining (PAS), Masson’s trichrome staining (MASSON) and Verhoeff-van Gieson staining (VG) are shown. Black arrow represents glycogen deposition, green arrows represent muscle fiber hyperplasia. c X-ray autoradiographs of hindlimbs and forelimbs of 8-month-old WT and GCK-Hom rabbit. The blue arrows represent compact bone substance in rabbits. The Red arrows represent bone density. d BMD of the Fibula and tibia were analyzed in WT and GCK-Hom rabbit. e Quantification of the compact bone substance thickness. *p < 0.05, **p < 0.01 vs. age-matched WT rabbits. f, g Pathological section of the fibula (f) and osteoarticular (g) in WT and GCK-Hom rabbit