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Generation of genetically GCK-targeted rabbits using CRISPR/Cas9
| gRNA/Cas9 mRNA (ng/uL) | Embryos injected | Transferred embryos (% microinjected) | Recipients | Pups obtained (% transferred) | Mutations in the individual (% pups) | Homozygous mutations (% pups) | |
|---|---|---|---|---|---|---|---|
| 1 | 25/100 | 40 | 30 (75%) | No | |||
| 2 | 25/100 | 45 | 40 (89%) | No | |||
| 3 | 25/100 | 40 | 34 (85%) | Yes | 5 (12.5%) | 4 (80%) | 1 (20%) |
| 4 | 25/100 | 45 | 42 (91%) | Yes | 7 (15%) | 6 (86%) | 2 (28.5%) |
