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sAC knockdown suppressed AMPK activity. Transfection of H9C2 cells with plasmids encoding either sAC-targeted (sAC shRNA) or scramble shRNA significantly reduced sAC expression (a), cellular cAMP content (b) and the phosphorylation of AMPK (c) and its direct target ACC (d). Data are means ± SEM. n = 10 for a, c, d and n = 6 for b. *P < 0.05 vs. scramble
